BAIT

PAM16

MIA1, TIM16, YJL104W
Subunit of the import motor (PAM complex); the PAM complex is a component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); forms a 1:1 subcomplex with Pam18p and inhibits its cochaperone activity; contains a J-like domain
GO Process (1)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

ATP11

L000000148, YNL315C
Molecular chaperone; required for the assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase; N-terminally propionylated in vivo
GO Process (2)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

The yeast magmas ortholog pam16 has an essential function in fermentative growth that involves sphingolipid metabolism.

Short MK, Hallett JP, Tar K, Dange T, Schmidt M, Moir R, Willis IM, Jubinsky PT

Magmas is a growth factor responsive gene encoding an essential mitochondrial protein in mammalian cells. Pam16, the Magmas ortholog in Saccharomyces cerevisiae, is a component of the presequence translocase-associated motor. A temperature-sensitive allele (pam16-I61N) was used to query an array of non-essential gene-deletion strains for synthetic genetic interactions. The pam16-I61N mutation at ambient temperature caused synthetic lethal or sick phenotypes ... [more]

PLoS ONE Jul. 19, 2012; 7(7);e39428 [Pubmed: 22808036]

Throughput

  • High Throughput

Ontology Terms

  • vegetative growth (APO:0000106)
  • heat sensitivity (APO:0000147)

Additional Notes

  • SGA: query strain is pam16-161N, temperature-sensitive
  • SUR4 deletion rescues synthetic growth defect

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ATP11 PAM16
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.2883BioGRID
2067100

Curated By

  • BioGRID