TAP2
Gene Ontology Biological Process
- antigen processing and presentation of endogenous peptide antigen via MHC class I [IDA, ISO]
- antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent [IBA, ISO]
- antigen processing and presentation of endogenous peptide antigen via MHC class Ib via ER pathway, TAP-dependent [IBA, ISO]
- antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent [IBA, ISO]
- cytosol to ER transport [ISO]
- intracellular transport of viral protein in host cell [ISO]
- peptide antigen transport [ISO, TAS]
- peptide transport [IMP]
- positive regulation of T cell mediated cytotoxicity [IDA, ISO]
- positive regulation of antigen processing and presentation of peptide antigen via MHC class I [IMP]
- protection from natural killer cell mediated cytotoxicity [IMP]
- protein complex assembly [IDA]
- response to molecule of bacterial origin [ISO]
- transmembrane transport [IBA]
Gene Ontology Molecular Function- ADP binding [IDA]
- ATP binding [IDA, IMP, ISO]
- ATPase activity, coupled to transmembrane movement of substances [IBA]
- MHC class I protein binding [IDA]
- MHC class Ib protein binding [ISO]
- TAP1 binding [IPI, ISO]
- TAP2 binding [IPI]
- nucleotide binding [IDA]
- peptide antigen binding [IDA]
- peptide antigen-transporting ATPase activity [ISO, TAS]
- peptide transporter activity [ISO]
- protein heterodimerization activity [IDA]
- protein homodimerization activity [IDA, IPI]
- tapasin binding [IDA, IPI]
- transporter activity [ISO]
- ADP binding [IDA]
- ATP binding [IDA, IMP, ISO]
- ATPase activity, coupled to transmembrane movement of substances [IBA]
- MHC class I protein binding [IDA]
- MHC class Ib protein binding [ISO]
- TAP1 binding [IPI, ISO]
- TAP2 binding [IPI]
- nucleotide binding [IDA]
- peptide antigen binding [IDA]
- peptide antigen-transporting ATPase activity [ISO, TAS]
- peptide transporter activity [ISO]
- protein heterodimerization activity [IDA]
- protein homodimerization activity [IDA, IPI]
- tapasin binding [IDA, IPI]
- transporter activity [ISO]
Gene Ontology Cellular Component
TAP1
Gene Ontology Biological Process
- ATP catabolic process [IDA]
- antigen processing and presentation of endogenous peptide antigen via MHC class I [ISO]
- cytosol to ER transport [ISO]
- defense response [ISO]
- intracellular transport of viral protein in host cell [ISO]
- peptide antigen transport [TAS]
- peptide transport [IMP, ISO]
- protection from natural killer cell mediated cytotoxicity [IMP]
- transmembrane transport [IBA]
Gene Ontology Molecular Function- ADP binding [IDA, ISO]
- ATP binding [IDA, ISO]
- ATPase activity [IDA]
- ATPase activity, coupled to transmembrane movement of substances [IBA]
- MHC class I protein binding [IDA, ISO]
- MHC class Ib protein binding [ISO]
- TAP1 binding [IPI]
- TAP2 binding [IPI, ISO]
- nucleotide binding [IDA]
- peptide antigen binding [IDA]
- peptide antigen-transporting ATPase activity [TAS]
- peptide transporter activity [ISO]
- protein heterodimerization activity [IDA]
- protein homodimerization activity [IPI]
- tapasin binding [IDA, IPI]
- ADP binding [IDA, ISO]
- ATP binding [IDA, ISO]
- ATPase activity [IDA]
- ATPase activity, coupled to transmembrane movement of substances [IBA]
- MHC class I protein binding [IDA, ISO]
- MHC class Ib protein binding [ISO]
- TAP1 binding [IPI]
- TAP2 binding [IPI, ISO]
- nucleotide binding [IDA]
- peptide antigen binding [IDA]
- peptide antigen-transporting ATPase activity [TAS]
- peptide transporter activity [ISO]
- protein heterodimerization activity [IDA]
- protein homodimerization activity [IPI]
- tapasin binding [IDA, IPI]
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Nucleotide binding by TAP mediates association with peptide and release of assembled MHC class I molecules.
Newly synthesised peptide-receptive major histocompatibility complex (MHC) class I molecules form a transient loading complex in the endoplasmic reticulum with the transporter associated with antigen processing (TAP) and a set of accessory proteins. Binding of peptide to the MHC class I molecule is necessary for dissociation of the MHC class I molecule from the complex with TAP, but other components ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| TAP1 TAP2 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID