BAIT
TAP2
Abcb3, Cim
transporter 2, ATP-binding cassette, sub-family B (MDR/TAP)
GO Process (14)
GO Function (15)
GO Component (6)
Gene Ontology Biological Process
- antigen processing and presentation of endogenous peptide antigen via MHC class I [IDA, ISO]
- antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent [IBA, ISO]
- antigen processing and presentation of endogenous peptide antigen via MHC class Ib via ER pathway, TAP-dependent [IBA, ISO]
- antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent [IBA, ISO]
- cytosol to ER transport [ISO]
- intracellular transport of viral protein in host cell [ISO]
- peptide antigen transport [ISO, TAS]
- peptide transport [IMP]
- positive regulation of T cell mediated cytotoxicity [IDA, ISO]
- positive regulation of antigen processing and presentation of peptide antigen via MHC class I [IMP]
- protection from natural killer cell mediated cytotoxicity [IMP]
- protein complex assembly [IDA]
- response to molecule of bacterial origin [ISO]
- transmembrane transport [IBA]
Gene Ontology Molecular Function- ADP binding [IDA]
- ATP binding [IDA, IMP, ISO]
- ATPase activity, coupled to transmembrane movement of substances [IBA]
- MHC class I protein binding [IDA]
- MHC class Ib protein binding [ISO]
- TAP1 binding [IPI, ISO]
- TAP2 binding [IPI]
- nucleotide binding [IDA]
- peptide antigen binding [IDA]
- peptide antigen-transporting ATPase activity [ISO, TAS]
- peptide transporter activity [ISO]
- protein heterodimerization activity [IDA]
- protein homodimerization activity [IDA, IPI]
- tapasin binding [IDA, IPI]
- transporter activity [ISO]
- ADP binding [IDA]
- ATP binding [IDA, IMP, ISO]
- ATPase activity, coupled to transmembrane movement of substances [IBA]
- MHC class I protein binding [IDA]
- MHC class Ib protein binding [ISO]
- TAP1 binding [IPI, ISO]
- TAP2 binding [IPI]
- nucleotide binding [IDA]
- peptide antigen binding [IDA]
- peptide antigen-transporting ATPase activity [ISO, TAS]
- peptide transporter activity [ISO]
- protein heterodimerization activity [IDA]
- protein homodimerization activity [IDA, IPI]
- tapasin binding [IDA, IPI]
- transporter activity [ISO]
Gene Ontology Cellular Component
Rattus norvegicus
PREY
CALR
CRT, HEL-S-99n, RO, SSA, cC1qR
calreticulin
GO Process (33)
GO Function (15)
GO Component (17)
Gene Ontology Biological Process
- activation of signaling protein activity involved in unfolded protein response [TAS]
- antigen processing and presentation of exogenous peptide antigen via MHC class I [TAS]
- antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent [TAS]
- antigen processing and presentation of peptide antigen via MHC class I [TAS]
- cell cycle arrest [IGI]
- cellular calcium ion homeostasis [TAS]
- cellular protein metabolic process [TAS]
- cellular senescence [IGI]
- endoplasmic reticulum unfolded protein response [TAS]
- glucocorticoid receptor signaling pathway [TAS]
- negative regulation of intracellular steroid hormone receptor signaling pathway [IDA]
- negative regulation of neuron differentiation [IDA]
- negative regulation of retinoic acid receptor signaling pathway [IDA]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- negative regulation of transcription, DNA-templated [IDA]
- negative regulation of translation [ISS, TAS]
- peptide antigen assembly with MHC class I protein complex [ISS]
- positive regulation of DNA replication [IGI]
- positive regulation of cell cycle [IGI]
- positive regulation of cell proliferation [IGI]
- positive regulation of dendritic cell chemotaxis [IMP]
- positive regulation of phagocytosis [ISS]
- positive regulation of substrate adhesion-dependent cell spreading [IMP]
- post-translational protein modification [TAS]
- protein N-linked glycosylation via asparagine [TAS]
- protein export from nucleus [IDA]
- protein folding [TAS]
- protein localization to nucleus [IDA]
- protein maturation by protein folding [TAS]
- protein stabilization [ISS, TAS]
- regulation of apoptotic process [TAS]
- regulation of transcription, DNA-templated [TAS]
- sequestering of calcium ion [TAS]
Gene Ontology Molecular Function- DNA binding [NAS]
- androgen receptor binding [IDA]
- calcium ion binding [ISS, TAS]
- carbohydrate binding [TAS]
- chaperone binding [TAS]
- complement component C1q binding [TAS]
- glycoprotein binding [IPI]
- integrin binding [IPI]
- mRNA binding [IDA]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- protein binding involved in protein folding [TAS]
- ubiquitin protein ligase binding [IPI]
- unfolded protein binding [TAS]
- zinc ion binding [TAS]
- DNA binding [NAS]
- androgen receptor binding [IDA]
- calcium ion binding [ISS, TAS]
- carbohydrate binding [TAS]
- chaperone binding [TAS]
- complement component C1q binding [TAS]
- glycoprotein binding [IPI]
- integrin binding [IPI]
- mRNA binding [IDA]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- protein binding involved in protein folding [TAS]
- ubiquitin protein ligase binding [IPI]
- unfolded protein binding [TAS]
- zinc ion binding [TAS]
Gene Ontology Cellular Component
- MHC class I peptide loading complex [ISS]
- cell surface [TAS]
- cytoplasm [IDA]
- cytosol [IDA]
- endocytic vesicle lumen [TAS]
- endoplasmic reticulum [IDA, TAS]
- endoplasmic reticulum lumen [IDA, TAS]
- extracellular region [TAS]
- extracellular space [IDA]
- extracellular vesicular exosome [IDA]
- focal adhesion [IDA]
- integral component of lumenal side of endoplasmic reticulum membrane [TAS]
- intracellular [TAS]
- membrane [IDA]
- nucleus [IDA]
- perinuclear region of cytoplasm [IDA]
- polysome [ISS]
Homo sapiens
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Nucleotide binding by TAP mediates association with peptide and release of assembled MHC class I molecules.
Newly synthesised peptide-receptive major histocompatibility complex (MHC) class I molecules form a transient loading complex in the endoplasmic reticulum with the transporter associated with antigen processing (TAP) and a set of accessory proteins. Binding of peptide to the MHC class I molecule is necessary for dissociation of the MHC class I molecule from the complex with TAP, but other components ... [more]
Curr. Biol. Sep. 23, 1999; 9(18);999-1008 [Pubmed: 10508608]
Throughput
- Low Throughput
Curated By
- BioGRID