BAIT

POU5F1

NF-A3, Oct-3, Oct-3/4, Oct-4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4
POU domain, class 5, transcription factor 1
GO Process (33)
GO Function (18)
GO Component (8)

Gene Ontology Biological Process

Mus musculus

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Oct4 links multiple epigenetic pathways to the pluripotency network.

Ding J, Xu H, Faiola F, Ma'ayan A, Wang J

Oct4 is a well-known transcription factor that plays fundamental roles in stem cell self-renewal, pluripotency, and somatic cell reprogramming. However, limited information is available on Oct4-associated protein complexes and their intrinsic protein-protein interactions that dictate Oct4's critical regulatory activities. Here we employed an improved affinity purification approach combined with mass spectrometry to purify Oct4 protein complexes in mouse embryonic stem ... [more]

Cell Res. Jan. 01, 2012; 22(1);155-67 [Pubmed: 22083510]

Throughput

  • High Throughput

Additional Notes

  • table S1.

Curated By

  • BioGRID