POU5F1
Gene Ontology Biological Process
- BMP signaling pathway involved in heart induction [ISO]
- blastocyst growth [IGI]
- cardiac cell fate determination [ISO]
- cell fate commitment [IMP]
- cell fate commitment involved in formation of primary germ layer [ISO]
- ectodermal cell fate commitment [IDA]
- endodermal cell fate commitment [IDA]
- endodermal cell fate specification [ISO]
- germ-line stem cell maintenance [IMP]
- mRNA transcription from RNA polymerase II promoter [IDA]
- mesodermal cell fate commitment [IDA]
- negative regulation of calcium ion-dependent exocytosis [IDA]
- negative regulation of cell differentiation [IGI]
- negative regulation of gene silencing by miRNA [ISO]
- negative regulation of protein kinase B signaling [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IGI]
- negative regulation of transcription, DNA-templated [IDA]
- positive regulation of SMAD protein import into nucleus [ISO]
- positive regulation of catenin import into nucleus [ISO]
- positive regulation of protein kinase B signaling [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IGI, IMP, ISO]
- positive regulation of transcription, DNA-templated [IDA]
- regulation of gene expression [ISO]
- regulation of heart induction by regulation of canonical Wnt signaling pathway [ISO]
- regulation of methylation-dependent chromatin silencing [ISO]
- regulation of transcription, DNA-templated [IDA, ISO]
- response to organic substance [IDA]
- response to retinoic acid [IDA]
- somatic stem cell maintenance [ISO]
- stem cell differentiation [IDA]
- stem cell maintenance [IMP]
- transcription from RNA polymerase II promoter [IDA, ISO]
- trophectodermal cell differentiation [IGI]
Gene Ontology Molecular Function- DNA binding [IDA, IGI, ISO]
- HMG box domain binding [ISO]
- POU domain binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- RNA polymerase II transcription coactivator activity [ISO]
- chromatin binding [IDA]
- miRNA binding [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- protein heterodimerization activity [IDA]
- sequence-specific DNA binding [IDA, ISO]
- sequence-specific DNA binding RNA polymerase II transcription factor activity [IDA]
- sequence-specific DNA binding transcription factor activity [IDA, ISO]
- transcription corepressor activity [IDA]
- transcription factor binding [IPI, ISO]
- transcription regulatory region DNA binding [IDA, ISO]
- ubiquitin protein ligase binding [IPI, ISO]
- DNA binding [IDA, IGI, ISO]
- HMG box domain binding [ISO]
- POU domain binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- RNA polymerase II transcription coactivator activity [ISO]
- chromatin binding [IDA]
- miRNA binding [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- protein heterodimerization activity [IDA]
- sequence-specific DNA binding [IDA, ISO]
- sequence-specific DNA binding RNA polymerase II transcription factor activity [IDA]
- sequence-specific DNA binding transcription factor activity [IDA, ISO]
- transcription corepressor activity [IDA]
- transcription factor binding [IPI, ISO]
- transcription regulatory region DNA binding [IDA, ISO]
- ubiquitin protein ligase binding [IPI, ISO]
ARID3B
Gene Ontology Biological Process
Gene Ontology Molecular Function- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Oct4 links multiple epigenetic pathways to the pluripotency network.
Oct4 is a well-known transcription factor that plays fundamental roles in stem cell self-renewal, pluripotency, and somatic cell reprogramming. However, limited information is available on Oct4-associated protein complexes and their intrinsic protein-protein interactions that dictate Oct4's critical regulatory activities. Here we employed an improved affinity purification approach combined with mass spectrometry to purify Oct4 protein complexes in mouse embryonic stem ... [more]
Throughput
- Low Throughput
Additional Notes
- figure 4B.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| POU5F1 ARID3B | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 674272 | |
| POU5F1 ARID3B | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | 471757 | |
| POU5F1 ARID3B | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| POU5F1 ARID3B | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| POU5F1 ARID3B | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | Low | - | BioGRID | 674469 |
Curated By
- BioGRID