BAIT

HIST2H2BE

GL105, H2B, H2B.1, H2BFQ, H2BGL105, H2BQ
histone cluster 2, H2be
Homo sapiens
PREY

TCF20

AR1, SPBP, TCF-20, RP4-669P10.13-001
transcription factor 20 (AR1)
Autism spectrum disorder (ASD) Project
GO Process (1)
GO Function (3)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Identification of two independent nucleosome-binding domains in the transcriptional co-activator SPBP.

Darvekar S, Johnsen SS, Eriksen AB, Johansen T, Sjottem E

Transcriptional regulation requires co-ordinated action of transcription factors, co-activator complexes and general transcription factors to access specific loci in the dense chromatin structure. In the present study we demonstrate that the transcriptional co-regulator SPBP [stromelysin-1 PDGF (platelet-derived growth factor)-responsive element binding protein] contains two independent chromatin-binding domains, the SPBP-(1551-1666) region and the C-terminal extended PHD [ePHD/ADD (extended plant homeodomain/ATRX-DNMT3-DNMT3L)] domain. ... [more]

Biochem. J. Feb. 15, 2012; 442(1);65-75 [Pubmed: 22081970]

Throughput

  • Low Throughput

Additional Notes

  • figure 3E.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
HIST2H2BE TCF20
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
3357792

Curated By

  • BioGRID