Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The interaction of Epac1 and Ran promotes Rap1 activation at the nuclear envelope.

Liu C, Takahashi M, Li Y, Dillon TJ, Kaech S, Stork PJ

Epac1 (exchange protein directly activated by cyclic AMP [cAMP]) couples intracellular cAMP to the activation of Rap1, a Ras family GTPase that regulates cell adhesion, proliferation, and differentiation. Using mass spectrometry, we identified the small G protein Ran and Ran binding protein 2 (RanBP2) as potential binding partners of Epac1. Ran is a small G protein best known for its ... [more]

Mol. Cell. Biol. Aug. 01, 2010; 30(16);3956-69 [Pubmed: 20547757]

Throughput

  • Low Throughput

Curated By

  • BioGRID