UBE2I
Gene Ontology Biological Process
- cellular protein metabolic process [TAS]
- cellular protein modification process [TAS]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- negative regulation of transcription, DNA-templated [IDA]
- post-translational protein modification [TAS]
- protein sumoylation [IDA, TAS]
- protein ubiquitination [IBA]
- ubiquitin-dependent protein catabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
SOX4
Gene Ontology Biological Process
- DNA damage response, detection of DNA damage [IDA]
- DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest [IMP]
- T cell differentiation [ISS]
- ascending aorta morphogenesis [ISS]
- atrial septum primum morphogenesis [ISS]
- canonical Wnt signaling pathway [ISS]
- cardiac right ventricle morphogenesis [ISS]
- cardiac ventricle formation [ISS]
- cellular response to glucose stimulus [ISS]
- glial cell development [ISS]
- glial cell proliferation [ISS]
- glucose homeostasis [ISS]
- heart development [ISS]
- kidney morphogenesis [ISS]
- limb bud formation [ISS]
- mitral valve morphogenesis [ISS]
- negative regulation of cell death [ISS]
- negative regulation of cell proliferation [IMP]
- negative regulation of protein export from nucleus [IMP]
- negative regulation of protein ubiquitination [IMP]
- neural tube formation [ISS]
- neuroepithelial cell differentiation [ISS]
- noradrenergic neuron differentiation [ISS]
- positive regulation of N-terminal peptidyl-lysine acetylation [IDA]
- positive regulation of Wnt signaling pathway [ISS]
- positive regulation of apoptotic process [IMP]
- positive regulation of canonical Wnt signaling pathway [ISS]
- positive regulation of cell proliferation [IMP]
- positive regulation of insulin secretion [ISS]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IMP]
- positive regulation of transcription, DNA-templated [IDA, IMP]
- positive regulation of translation [IMP]
- pro-B cell differentiation [ISS]
- protein stabilization [IMP]
- regulation of protein stability [IMP]
- regulation of transcription, DNA-templated [IDA]
- skeletal system development [ISS]
- spinal cord development [ISS]
- spinal cord motor neuron differentiation [ISS]
- sympathetic nervous system development [ISS]
- ventricular septum morphogenesis [ISS]
Gene Ontology Molecular Function- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- RNA polymerase II transcription coactivator activity [ISS]
- core promoter sequence-specific DNA binding [IDA]
- nucleic acid binding transcription factor activity [IMP]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription regulatory region sequence-specific DNA binding [ISS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- RNA polymerase II transcription coactivator activity [ISS]
- core promoter sequence-specific DNA binding [IDA]
- nucleic acid binding transcription factor activity [IMP]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription regulatory region sequence-specific DNA binding [ISS]
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Ubc9 interacts with SOX4 and represses its transcriptional activity.
SOX4 is a member of SOX transcriptional factor family that is crucial for many cellular processes. In this study, a yeast two-hybrid screening of human mammary cDNA library identified human ubiquitin-conjugating enzyme 9 (hUbc9) that interacted with SOX4. This interaction was confirmed by GST pull-down in vitro and co-immunoprecipitation assays in vivo. Deletion mapping demonstrated that HMG-box domain of SOX4 ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SOX4 UBE2I | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| UBE2I SOX4 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID