BAIT

YOP1

YIP2, L000004674, YPR028W
Membrane protein that interacts with Yip1p to mediate membrane traffic; interacts with Sey1p to maintain ER morphology; overexpression leads to cell death and accumulation of internal cell membranes; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; forms ER foci upon DNA replication stress
Saccharomyces cerevisiae (S288c)
PREY

POM152

L000001464, YMR129W
Glycoprotein subunit of transmembrane ring of nuclear pore complex; contributes to nucleocytoplasmic transport, nuclear pore complex (NPC) biogenesis and spindle pole body duplication; homologous to human NUP210
Saccharomyces cerevisiae (S288c)

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Integrity and Function of the Saccharomyces cerevisiae Spindle Pole Body Depends on Connections between the Membrane Proteins Ndc1, Rtn1, and Yop1.

Casey AK, Dawson TR, Chen J, Friederichs JM, Jaspersen SL, Wente SR

The nuclear envelope in Saccharomyces cerevisiae harbors two essential macromolecular protein assemblies: the nuclear pore complexes (NPCs) that enable nucleocytoplasmic transport, and the spindle pole bodies (SPBs) that mediate chromosome segregation. Previously, based on metazoan and budding yeast studies, we reported that reticulons and Yop1/DP1 play a role in the early steps of de novo NPC assembly. Here, we examined ... [more]

Unknown Jul. 13, 2012; 0(0); [Pubmed: 22798490]

Throughput

  • Low Throughput

Additional Notes

  • split-ubiquitin

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
YOP1 POM152
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Low-BioGRID
353107

Curated By

  • BioGRID