BAIT
RAD23B
RAD23 homolog B (S. cerevisiae)
GO Process (5)
GO Function (1)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- XPC complex [ISO, ISS]
- cytoplasm [ISO]
- nucleoplasm [ISO]
Rattus norvegicus
PREY
EEF2
Ef-2
eukaryotic translation elongation factor 2
GO Process (5)
GO Function (5)
GO Component (8)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Isolation of mammalian 26S proteasomes and p97/VCP complexes using the ubiquitin-like domain from HHR23B reveals novel proteasome-associated proteins.
Recent studies, mainly in yeast, have identified various cofactors that associate with the 26S proteasome and appear to influence its function. To identify these proteins in different cells and physiological states, we developed a method to gently and rapidly isolate 26S proteasomes and associated proteins without the need for genetic modifications of the proteasome. This method is based on the ... [more]
Biochemistry Mar. 24, 2009; 48(11);2538-49 [Pubmed: 19182904]
Throughput
- Low Throughput
Curated By
- BioGRID