BAIT
RAD23B
RAD23 homolog B (S. cerevisiae)
GO Process (5)
GO Function (1)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- XPC complex [ISO, ISS]
- cytoplasm [ISO]
- nucleoplasm [ISO]
Rattus norvegicus
PREY
USP7
Hausp
ubiquitin specific peptidase 7 (herpes virus-associated)
GO Process (12)
GO Function (8)
GO Component (3)
Gene Ontology Biological Process
- histone deubiquitination [IBA]
- maintenance of DNA methylation [ISO, ISS]
- negative regulation of NF-kappaB transcription factor activity [ISO]
- positive regulation of apoptotic process [IDA]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IBA]
- protein deubiquitination [IDA, ISO, ISS]
- proteolysis [ISO]
- regulation of proteasomal protein catabolic process [IBA]
- regulation of protein stability [IDA]
- regulation of sequence-specific DNA binding transcription factor activity [ISO, ISS]
- regulation of transcription, DNA-templated [IBA]
- transcription-coupled nucleotide-excision repair [IBA, ISO, ISS]
Gene Ontology Molecular Function- cysteine-type endopeptidase activity [IBA, ISO, ISS]
- p53 binding [ISO]
- protein C-terminus binding [ISO]
- protein homodimerization activity [IDA]
- transcription factor binding [ISO]
- ubiquitin protein ligase binding [ISO]
- ubiquitin thiolesterase activity [IBA, ISO, ISS]
- ubiquitin-specific protease activity [IDA, ISO, ISS]
- cysteine-type endopeptidase activity [IBA, ISO, ISS]
- p53 binding [ISO]
- protein C-terminus binding [ISO]
- protein homodimerization activity [IDA]
- transcription factor binding [ISO]
- ubiquitin protein ligase binding [ISO]
- ubiquitin thiolesterase activity [IBA, ISO, ISS]
- ubiquitin-specific protease activity [IDA, ISO, ISS]
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Isolation of mammalian 26S proteasomes and p97/VCP complexes using the ubiquitin-like domain from HHR23B reveals novel proteasome-associated proteins.
Recent studies, mainly in yeast, have identified various cofactors that associate with the 26S proteasome and appear to influence its function. To identify these proteins in different cells and physiological states, we developed a method to gently and rapidly isolate 26S proteasomes and associated proteins without the need for genetic modifications of the proteasome. This method is based on the ... [more]
Biochemistry Mar. 24, 2009; 48(11);2538-49 [Pubmed: 19182904]
Throughput
- Low Throughput
Curated By
- BioGRID