BAIT
RAD23B
RAD23 homolog B (S. cerevisiae)
GO Process (5)
GO Function (1)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- XPC complex [ISO, ISS]
- cytoplasm [ISO]
- nucleoplasm [ISO]
Rattus norvegicus
PREY
VIM
vimentin
GO Process (21)
GO Function (12)
GO Component (16)
Gene Ontology Biological Process
- Bergmann glial cell differentiation [ISO]
- SMAD protein signal transduction [ISO]
- aging [IEP]
- astrocyte development [ISO]
- cellular response to fibroblast growth factor stimulus [IEP]
- decidualization [IEP]
- fat cell differentiation [IEP]
- intermediate filament organization [ISO]
- intermediate filament-based process [ISO]
- lens fiber cell development [ISO]
- negative regulation of neuron projection development [ISO]
- positive regulation of gene expression [ISO]
- positive regulation of glial cell proliferation [IMP]
- regulation of Schwann cell migration [IDA]
- regulation of axonogenesis [IDA]
- response to estradiol [IEP]
- response to radiation [IEP]
- response to selenium ion [IEP]
- response to vitamin D [IEP]
- response to zinc ion [IEP]
- skeletal muscle tissue regeneration [IEP]
Gene Ontology Molecular Function- double-stranded RNA binding [ISO]
- glycoprotein binding [ISO]
- identical protein binding [ISO]
- kinase binding [IPI]
- protein C-terminus binding [ISO]
- protein binding [IPI]
- protein kinase binding [IPI]
- protein phosphatase 2A binding [IDA]
- scaffold protein binding [ISO]
- structural constituent of cytoskeleton [ISO, ISS]
- structural constituent of eye lens [ISO]
- structural molecule activity [ISO, TAS]
- double-stranded RNA binding [ISO]
- glycoprotein binding [ISO]
- identical protein binding [ISO]
- kinase binding [IPI]
- protein C-terminus binding [ISO]
- protein binding [IPI]
- protein kinase binding [IPI]
- protein phosphatase 2A binding [IDA]
- scaffold protein binding [ISO]
- structural constituent of cytoskeleton [ISO, ISS]
- structural constituent of eye lens [ISO]
- structural molecule activity [ISO, TAS]
Gene Ontology Cellular Component
- axon [IDA]
- cell body [IDA]
- cell leading edge [ISO]
- cell projection [IDA, ISO]
- cytoplasm [IDA, ISO, ISS]
- cytoskeleton [IDA, ISO]
- cytosol [IDA, ISO]
- extracellular vesicular exosome [ISO]
- focal adhesion [ISO]
- intermediate filament [ISO, ISS]
- intermediate filament cytoskeleton [ISO]
- neuron projection [ISO]
- perinuclear region of cytoplasm [IDA]
- peroxisome [ISO]
- plasma membrane [ISO]
- type III intermediate filament [TAS]
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Isolation of mammalian 26S proteasomes and p97/VCP complexes using the ubiquitin-like domain from HHR23B reveals novel proteasome-associated proteins.
Recent studies, mainly in yeast, have identified various cofactors that associate with the 26S proteasome and appear to influence its function. To identify these proteins in different cells and physiological states, we developed a method to gently and rapidly isolate 26S proteasomes and associated proteins without the need for genetic modifications of the proteasome. This method is based on the ... [more]
Biochemistry Mar. 24, 2009; 48(11);2538-49 [Pubmed: 19182904]
Throughput
- Low Throughput
Curated By
- BioGRID