BAIT
RAD23B
RAD23 homolog B (S. cerevisiae)
GO Process (5)
GO Function (1)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- XPC complex [ISO, ISS]
- cytoplasm [ISO]
- nucleoplasm [ISO]
Rattus norvegicus
PREY
ANXA1
Anx1, p35
annexin A1
GO Process (39)
GO Function (12)
GO Component (14)
Gene Ontology Biological Process
- ATP catabolic process [IMP]
- DNA duplex unwinding [IMP]
- DNA rewinding [IMP]
- DNA strand renaturation [IMP]
- alpha-beta T cell differentiation [ISO]
- arachidonic acid secretion [ISO]
- cell surface receptor signaling pathway [IDA]
- cellular response to glucocorticoid stimulus [IDA, ISO]
- cellular response to hydrogen peroxide [IMP]
- endocrine pancreas development [IEP]
- estrous cycle phase [IEP]
- gliogenesis [IEP]
- hepatocyte differentiation [IEP]
- insulin secretion [IDA]
- keratinocyte differentiation [ISO]
- negative regulation of interleukin-8 secretion [ISO]
- negative regulation of phospholipase A2 activity [IDA]
- negative regulation of protein secretion [IMP]
- neutrophil clearance [ISO]
- neutrophil homeostasis [ISO]
- peptide cross-linking [ISO]
- positive regulation of G1/S transition of mitotic cell cycle [ISO]
- positive regulation of apoptotic process [IMP]
- positive regulation of neutrophil apoptotic process [ISO]
- positive regulation of prostaglandin biosynthetic process [IMP]
- positive regulation of vesicle fusion [ISO]
- prolactin secretion [IMP]
- prostate gland development [IEP]
- regulation of cell proliferation [ISO]
- response to X-ray [IEP]
- response to corticosteroid [IEP]
- response to drug [IEP]
- response to estradiol [IEP]
- response to glucocorticoid [IEP]
- response to hormone [IEP]
- response to interleukin-1 [IEP]
- response to organic cyclic compound [IEP]
- response to peptide hormone [IEP]
- signal transduction [ISO]
Gene Ontology Molecular Function- annealing helicase activity [IMP]
- calcium-dependent phospholipid binding [ISO, TAS]
- calcium-dependent protein binding [ISO]
- double-stranded DNA-dependent ATPase activity [IMP]
- helicase activity [IMP]
- phospholipase A2 inhibitor activity [IDA, ISO]
- phospholipid binding [IDA]
- protein binding, bridging [ISO]
- protein homodimerization activity [IDA]
- single-stranded DNA binding [IDA]
- single-stranded RNA binding [IDA]
- structural molecule activity [ISO]
- annealing helicase activity [IMP]
- calcium-dependent phospholipid binding [ISO, TAS]
- calcium-dependent protein binding [ISO]
- double-stranded DNA-dependent ATPase activity [IMP]
- helicase activity [IMP]
- phospholipase A2 inhibitor activity [IDA, ISO]
- phospholipid binding [IDA]
- protein binding, bridging [ISO]
- protein homodimerization activity [IDA]
- single-stranded DNA binding [IDA]
- single-stranded RNA binding [IDA]
- structural molecule activity [ISO]
Gene Ontology Cellular Component
- cell surface [ISO]
- cornified envelope [ISO]
- cytoplasm [IDA, ISO]
- endosome [ISO]
- extracellular space [IDA, ISO]
- extracellular vesicular exosome [ISO]
- focal adhesion [ISO]
- mast cell granule [IDA]
- mitochondrial membrane [IDA]
- nucleus [IDA, ISO]
- plasma membrane [IDA, ISO]
- protein complex [IDA]
- sarcolemma [ISO]
- vesicle [ISO]
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Isolation of mammalian 26S proteasomes and p97/VCP complexes using the ubiquitin-like domain from HHR23B reveals novel proteasome-associated proteins.
Recent studies, mainly in yeast, have identified various cofactors that associate with the 26S proteasome and appear to influence its function. To identify these proteins in different cells and physiological states, we developed a method to gently and rapidly isolate 26S proteasomes and associated proteins without the need for genetic modifications of the proteasome. This method is based on the ... [more]
Biochemistry Mar. 24, 2009; 48(11);2538-49 [Pubmed: 19182904]
Throughput
- Low Throughput
Curated By
- BioGRID