BAIT
RAD23B
RAD23 homolog B (S. cerevisiae)
GO Process (5)
GO Function (1)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- XPC complex [ISO, ISS]
- cytoplasm [ISO]
- nucleoplasm [ISO]
Rattus norvegicus
PREY
ANXA2
ANX2
annexin A2
GO Process (15)
GO Function (9)
GO Component (24)
Gene Ontology Biological Process
- angiogenesis [ISO]
- body fluid secretion [IMP]
- collagen fibril organization [ISO]
- fibrinolysis [ISO]
- membrane budding [ISO]
- membrane raft assembly [ISO]
- negative regulation of catalytic activity [ISO]
- osteoclast development [ISO]
- positive regulation of binding [ISO]
- positive regulation of fibroblast proliferation [IMP]
- positive regulation of protein phosphorylation [IMP]
- positive regulation of vesicle fusion [ISO]
- protein heterotetramerization [ISO]
- protein targeting to plasma membrane [IMP]
- regulation of fibrinolysis [TAS]
Gene Ontology Molecular Function- Rab GTPase binding [IPI]
- S100 protein binding [ISO]
- actin filament binding [TAS]
- calcium ion binding [TAS]
- calcium-dependent phospholipid binding [ISO]
- calcium-dependent protein binding [ISO]
- phosphatidylinositol-4,5-bisphosphate binding [IDA, ISO]
- phospholipase A2 inhibitor activity [ISO]
- poly(A) RNA binding [ISO]
- Rab GTPase binding [IPI]
- S100 protein binding [ISO]
- actin filament binding [TAS]
- calcium ion binding [TAS]
- calcium-dependent phospholipid binding [ISO]
- calcium-dependent protein binding [ISO]
- phosphatidylinositol-4,5-bisphosphate binding [IDA, ISO]
- phospholipase A2 inhibitor activity [ISO]
- poly(A) RNA binding [ISO]
Gene Ontology Cellular Component
- Schmidt-Lanterman incisure [ISO]
- cell cortex [IDA]
- cell surface [ISO]
- cytoplasm [IDA, ISO]
- early endosome [ISO]
- endosome [ISO]
- extracellular space [ISO]
- extracellular vesicular exosome [ISO]
- extrinsic component of plasma membrane [ISO]
- late endosome membrane [ISO]
- lipid particle [ISO]
- lysosomal membrane [ISO]
- macropinosome [IDA]
- membrane [ISO]
- membrane raft [ISO]
- midbody [ISO]
- myelin sheath adaxonal region [ISO]
- nucleus [ISO]
- perinuclear region of cytoplasm [IDA]
- plasma membrane [IDA, ISO]
- protein complex [IDA]
- ruffle [IDA]
- sarcolemma [ISO]
- vesicle [IDA, ISO]
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Isolation of mammalian 26S proteasomes and p97/VCP complexes using the ubiquitin-like domain from HHR23B reveals novel proteasome-associated proteins.
Recent studies, mainly in yeast, have identified various cofactors that associate with the 26S proteasome and appear to influence its function. To identify these proteins in different cells and physiological states, we developed a method to gently and rapidly isolate 26S proteasomes and associated proteins without the need for genetic modifications of the proteasome. This method is based on the ... [more]
Biochemistry Mar. 24, 2009; 48(11);2538-49 [Pubmed: 19182904]
Throughput
- Low Throughput
Curated By
- BioGRID