BAIT
GRIN2B
AW490526, GluN2B, NR2B, Nmdar2b
glutamate receptor, ionotropic, NMDA2B (epsilon 2)
GO Process (35)
GO Function (17)
GO Component (14)
Gene Ontology Biological Process
- action potential [ISO]
- associative learning [ISO]
- behavioral fear response [IMP]
- behavioral response to pain [IMP]
- calcium ion transmembrane transport [IMP]
- calcium ion transport [IMP]
- cation transmembrane transport [IGI]
- cation transport [IGI]
- detection of mechanical stimulus involved in sensory perception of pain [IMP]
- embryo development [IMP]
- fear response [IMP]
- in utero embryonic development [IMP]
- ion transmembrane transport [IBA, ISO]
- ionotropic glutamate receptor signaling pathway [IGI, ISO]
- learning [IDA, IMP]
- learning or memory [TAS]
- memory [IDA, IMP, ISO]
- positive regulation of cell death [ISO]
- positive regulation of glutamate secretion [ISO]
- positive regulation of synaptic transmission [ISO]
- receptor clustering [ISO]
- regulation of MAPK cascade [ISO]
- regulation of excitatory postsynaptic membrane potential [IGI, IMP]
- regulation of long-term neuronal synaptic plasticity [ISO]
- regulation of neuronal synaptic plasticity [TAS]
- regulation of postsynaptic membrane potential [IMP]
- regulation of protein kinase A signaling [IMP]
- regulation of synaptic plasticity [IMP]
- response to ethanol [IMP, ISO]
- rhythmic process [ISO]
- sensory organ development [IMP]
- startle response [IGI, IMP]
- suckling behavior [IMP]
- synaptic transmission [IMP]
- synaptic transmission, glutamatergic [IBA]
Gene Ontology Molecular Function- D2 dopamine receptor binding [ISO]
- N-methyl-D-aspartate selective glutamate receptor activity [IGI, IMP, ISO]
- beta-catenin binding [ISO]
- calcium channel activity [IMP]
- cation channel activity [IGI]
- cell adhesion molecule binding [ISO]
- drug binding [ISO]
- extracellular-glutamate-gated ion channel activity [IBA, ISO]
- glycine binding [ISO]
- interleukin-1 receptor binding [ISO]
- ionotropic glutamate receptor activity [ISO]
- ionotropic glutamate receptor binding [ISO]
- neurotransmitter binding [ISO]
- protein binding [IPI]
- protein heterodimerization activity [ISO]
- receptor binding [ISO]
- zinc ion binding [ISO]
- D2 dopamine receptor binding [ISO]
- N-methyl-D-aspartate selective glutamate receptor activity [IGI, IMP, ISO]
- beta-catenin binding [ISO]
- calcium channel activity [IMP]
- cation channel activity [IGI]
- cell adhesion molecule binding [ISO]
- drug binding [ISO]
- extracellular-glutamate-gated ion channel activity [IBA, ISO]
- glycine binding [ISO]
- interleukin-1 receptor binding [ISO]
- ionotropic glutamate receptor activity [ISO]
- ionotropic glutamate receptor binding [ISO]
- neurotransmitter binding [ISO]
- protein binding [IPI]
- protein heterodimerization activity [ISO]
- receptor binding [ISO]
- zinc ion binding [ISO]
Gene Ontology Cellular Component
- N-methyl-D-aspartate selective glutamate receptor complex [IPI, ISO]
- Z disc [ISO]
- cell surface [IDA]
- dendritic spine [ISO]
- membrane [IDA]
- neuron projection [IDA, ISO]
- neuronal postsynaptic density [IDA]
- postsynaptic density [IDA, ISO]
- postsynaptic membrane [IDA]
- presynaptic membrane [IDA, ISO]
- synapse [IDA, ISO]
- synaptic cleft [ISO]
- synaptic vesicle [IDA]
- terminal bouton [ISO]
Mus musculus
PREY
PIK3R1
PI3K, p50alpha, p55alpha, p85alpha
phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha)
GO Process (39)
GO Function (23)
GO Component (11)
Gene Ontology Biological Process
- B cell differentiation [IMP]
- NFAT protein import into nucleus [IMP]
- cellular glucose homeostasis [IDA]
- cellular response to UV [IGI, IMP]
- cellular response to insulin stimulus [IDA]
- extrinsic apoptotic signaling pathway via death domain receptors [IGI, IMP]
- glucose metabolic process [ISO]
- growth hormone receptor signaling pathway [ISO]
- insulin receptor signaling pathway [ISO]
- insulin-like growth factor receptor signaling pathway [ISO]
- intrinsic apoptotic signaling pathway in response to DNA damage [IGI, IMP]
- negative regulation of apoptotic process [IDA, ISO]
- negative regulation of blood pressure [ISO]
- negative regulation of cell-cell adhesion [ISO]
- negative regulation of cell-matrix adhesion [IGI]
- negative regulation of heart rate [ISO]
- negative regulation of muscle cell apoptotic process [ISO]
- negative regulation of osteoclast differentiation [IGI]
- negative regulation of proteolysis [ISO]
- negative regulation of smooth muscle cell proliferation [ISO]
- phosphatidylinositol 3-kinase signaling [ISO]
- phosphatidylinositol phosphorylation [TAS]
- phosphatidylinositol-3-phosphate biosynthetic process [ISO, TAS]
- positive regulation of RNA splicing [ISO]
- positive regulation of cell migration [IGI, ISO]
- positive regulation of endoplasmic reticulum unfolded protein response [ISO]
- positive regulation of gene expression [ISO]
- positive regulation of myoblast differentiation [ISO]
- positive regulation of protein phosphorylation [ISO]
- positive regulation of transcription factor import into nucleus [IMP, ISO]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IMP, ISO]
- positive regulation of tumor necrosis factor production [IMP]
- protein phosphorylation [IDA]
- protein stabilization [ISO]
- regulation of phosphatidylinositol 3-kinase activity [ISO]
- response to cAMP [ISO]
- response to endoplasmic reticulum stress [IDA, ISO]
- response to glucocorticoid [ISO]
- response to insulin [ISO]
Gene Ontology Molecular Function- 1-phosphatidylinositol-3-kinase activity [ISO, TAS]
- 1-phosphatidylinositol-3-kinase regulator activity [ISO]
- ATPase binding [ISO]
- ErbB-3 class receptor binding [ISO]
- calmodulin binding [ISO]
- estrogen receptor binding [ISO]
- insulin binding [ISO]
- insulin receptor binding [ISO]
- insulin receptor substrate binding [IPI, ISO]
- insulin-like growth factor receptor binding [ISO]
- neurotrophin TRKA receptor binding [ISO]
- phosphatidylinositol 3-kinase binding [ISO]
- phosphoprotein binding [ISO]
- platelet-derived growth factor receptor binding [ISO]
- protein C-terminus binding [ISO]
- protein binding [IPI]
- protein domain specific binding [ISO]
- protein kinase binding [ISO]
- protein phosphatase binding [ISO]
- receptor binding [ISO]
- receptor tyrosine kinase binding [ISO]
- transcription factor binding [IPI, ISO]
- ubiquitin protein ligase binding [ISO]
- 1-phosphatidylinositol-3-kinase activity [ISO, TAS]
- 1-phosphatidylinositol-3-kinase regulator activity [ISO]
- ATPase binding [ISO]
- ErbB-3 class receptor binding [ISO]
- calmodulin binding [ISO]
- estrogen receptor binding [ISO]
- insulin binding [ISO]
- insulin receptor binding [ISO]
- insulin receptor substrate binding [IPI, ISO]
- insulin-like growth factor receptor binding [ISO]
- neurotrophin TRKA receptor binding [ISO]
- phosphatidylinositol 3-kinase binding [ISO]
- phosphoprotein binding [ISO]
- platelet-derived growth factor receptor binding [ISO]
- protein C-terminus binding [ISO]
- protein binding [IPI]
- protein domain specific binding [ISO]
- protein kinase binding [ISO]
- protein phosphatase binding [ISO]
- receptor binding [ISO]
- receptor tyrosine kinase binding [ISO]
- transcription factor binding [IPI, ISO]
- ubiquitin protein ligase binding [ISO]
Gene Ontology Cellular Component
Mus musculus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Proteomic analysis of NMDA receptor-adhesion protein signaling complexes.
N-methyl-d-aspartate receptors (NMDAR) mediate long-lasting changes in synapse strength via downstream signaling pathways. We report proteomic characterization with mass spectrometry and immunoblotting of NMDAR multiprotein complexes (NRC) isolated from mouse brain. The NRC comprised 77 proteins organized into receptor, adaptor, signaling, cytoskeletal and novel proteins, of which 30 are implicated from binding studies and another 19 participate in NMDAR signaling. ... [more]
Nat. Neurosci. Jul. 01, 2000; 3(7);661-9 [Pubmed: 10862698]
Throughput
- Low Throughput
Curated By
- BioGRID