BAIT

NEDD8

NEDD-8
neural precursor cell expressed, developmentally down-regulated 8
UPS Project
Homo sapiens
PREY

MEI1

SPATA38, RP5-821D11.2
meiosis inhibitor 1
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Analysis of Nedd8-associated polypeptides: a model for deciphering the pathway for ubiquitin-like modifications.

Norman JA, Shiekhattar R

Ubiquitin-like proteins modify target proteins, altering their activities or causing them to be slated for degradation. These modifications are used to efficiently regulate key events in the cell. To explore the set of proteins modified by a small ubiquitin-like protein, we have developed a proteomic approach. Affinity purification of an epitope-tagged Nedd8 allowed the identification of the majority of proteins ... [more]

Biochemistry Mar. 07, 2006; 45(9);3014-9 [Pubmed: 16503656]

Throughput

  • Low Throughput

Additional Notes

  • #LPPI
  • Likely protein-protein interaction

Curated By

  • BioGRID