Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The multidomain protooncogenic protein c-Cbl binds to tubulin and stabilizes microtubules.

Teckchandani AM, Birukova AA, Tar K, Verin AD, Tsygankov AY

The protooncogenic protein c-Cbl is known to regulate the actin cytoskeleton. In this study, we present results indicating that c-Cbl can also regulate the microtubular network. We have shown that c-Cbl binds to tubulin and microtubules through its tyrosine kinase binding (TKB) domain. However, the character of the interactions described in this report is novel, since the G306E mutation, which ... [more]

Exp. Cell Res. May. 15, 2005; 306(1);114-27 [Pubmed: 15878338]

Throughput

  • Low Throughput

Curated By

  • BioGRID