BAIT

ERF1

ATERF1, ETHYLENE RESPONSE FACTOR 1, AT3G23240
ethylene-responsive transcription factor 1B
GO Process (4)
GO Function (2)
GO Component (1)
Arabidopsis thaliana (Columbia)
PREY

PFT1

F2J7.4, F2J7_4, PHYTOCHROME AND FLOWERING TIME 1, AT1G25540
mediator of RNA polymerase II transcription subunit 25
Arabidopsis thaliana (Columbia)

Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

MED25 Acts as an Integrative Hub for the Regulation of Jasmonate-Responsive Gene Expression in Arabidopsis.

Cevik V, Kidd BN, Zhang P, Hill C, Kiddle S, Denby KJ, Holub EB, Cahill DM, Manners JM, Schenk PM, Beynon J, Kazan K

The PHYTOCHROME AND FLOWERING TIME1 (PFT1) gene encoding the MEDIATOR25 (MED25) subunit of the eukaryotic Mediator complex is a positive regulator of jasmonate- (JA) responsive gene expression in Arabidopsis thaliana. Based on the function of the Mediator complex as a bridge between DNA-bound transcriptional activators and the RNA polymerase II complex, MED25 has been hypothesized to function in association with ... [more]

Unknown Jul. 22, 2012; 0(0); [Pubmed: 22822211]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ERF1 PFT1
PCA
PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Low-BioGRID
-
ERF1 PFT1
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
-
PFT1 ERF1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-
PFT1 ERF1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID