BAIT
RIPK1
D330015H01Rik, RIP, Rinp, Rip1
receptor (TNFRSF)-interacting serine-threonine kinase 1
GO Process (37)
GO Function (8)
GO Component (7)
Gene Ontology Biological Process
- T cell apoptotic process [IMP]
- amyloid fibril formation [ISO]
- apoptotic process [ISO]
- cellular protein catabolic process [ISO]
- cellular response to growth factor stimulus [IMP]
- cellular response to tumor necrosis factor [ISO]
- extrinsic apoptotic signaling pathway [ISO]
- necroptotic process [IGI, ISO]
- necroptotic signaling pathway [IMP, ISO]
- negative regulation of I-kappaB kinase/NF-kappaB signaling [ISO]
- negative regulation of extrinsic apoptotic signaling pathway [IGI, ISO]
- negative regulation of extrinsic apoptotic signaling pathway in absence of ligand [IMP]
- peptidyl-serine autophosphorylation [ISO]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [IMP, ISO]
- positive regulation of JNK cascade [IMP, ISO]
- positive regulation of MAPK cascade [IMP]
- positive regulation of NF-kappaB transcription factor activity [IMP, ISO]
- positive regulation of apoptotic process [ISO]
- positive regulation of cell death [ISO]
- positive regulation of extrinsic apoptotic signaling pathway [IGI, ISO]
- positive regulation of interleukin-8 production [ISO]
- positive regulation of macrophage differentiation [ISO]
- positive regulation of necroptotic process [ISO]
- positive regulation of phosphorylation [IMP]
- positive regulation of programmed cell death [ISO]
- positive regulation of protein phosphorylation [ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- positive regulation of transferase activity [IGI]
- positive regulation of tumor necrosis factor production [ISO]
- protein autophosphorylation [ISO]
- protein heterooligomerization [ISO]
- protein homooligomerization [ISO]
- regulation of ATP:ADP antiporter activity [ISO]
- regulation of reactive oxygen species metabolic process [IMP]
- response to tumor necrosis factor [ISO]
- ripoptosome assembly [ISO]
- ripoptosome assembly involved in necroptotic process [IGI]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
TRAF6
2310003F17Rik, AI851288, C630032O20Rik, RP23-313G3.1
TNF receptor-associated factor 6
GO Process (44)
GO Function (13)
GO Component (12)
Gene Ontology Biological Process
- I-kappaB kinase/NF-kappaB signaling [IMP]
- JNK cascade [ISO]
- T cell receptor signaling pathway [ISO]
- T-helper 1 type immune response [IMP]
- activation of NF-kappaB-inducing kinase activity [ISO]
- activation of protein kinase activity [ISO]
- antigen processing and presentation of exogenous peptide antigen via MHC class II [IMP]
- bone remodeling [IMP]
- bone resorption [IMP]
- cell development [IMP]
- cellular response to lipopolysaccharide [ISO]
- cytokine-mediated signaling pathway [ISO]
- immune response [IMP]
- interleukin-1-mediated signaling pathway [IMP, ISO]
- myeloid dendritic cell differentiation [IMP]
- negative regulation of transcription from RNA polymerase II promoter [ISO]
- negative regulation of transcription, DNA-templated [ISO]
- neural tube closure [IMP]
- odontogenesis of dentin-containing tooth [IMP]
- organ morphogenesis [IMP]
- ossification [IMP]
- osteoclast differentiation [IMP]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [IGI, IMP, ISO]
- positive regulation of JUN kinase activity [ISO]
- positive regulation of NF-kappaB transcription factor activity [IMP, ISO]
- positive regulation of T cell cytokine production [ISO]
- positive regulation of T cell proliferation [IMP]
- positive regulation of interleukin-12 biosynthetic process [IMP]
- positive regulation of interleukin-2 production [ISO]
- positive regulation of interleukin-6 biosynthetic process [IMP]
- positive regulation of lipopolysaccharide-mediated signaling pathway [IMP]
- positive regulation of osteoclast differentiation [ISO]
- positive regulation of sequence-specific DNA binding transcription factor activity [ISO]
- positive regulation of smooth muscle cell proliferation [ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- positive regulation of transcription regulatory region DNA binding [ISO]
- protein K63-linked ubiquitination [IDA, ISO]
- protein autoubiquitination [ISO, TAS]
- protein complex assembly [ISO]
- protein polyubiquitination [ISO]
- protein ubiquitination [IDA, IGI, IMP]
- regulation of immunoglobulin secretion [IDA]
- response to interleukin-1 [ISO]
- signal transduction [IDA, TAS]
Gene Ontology Molecular Function- histone deacetylase binding [ISO]
- mitogen-activated protein kinase kinase kinase binding [ISO]
- protein N-terminus binding [ISO]
- protein binding [IPI]
- protein kinase B binding [ISO]
- protein kinase binding [ISO]
- signal transducer activity [TAS]
- thioesterase binding [ISO]
- tumor necrosis factor receptor binding [ISO]
- ubiquitin conjugating enzyme binding [ISO]
- ubiquitin protein ligase activity [IDA, IMP]
- ubiquitin protein ligase binding [ISO]
- ubiquitin-protein transferase activity [IDA, ISO]
- histone deacetylase binding [ISO]
- mitogen-activated protein kinase kinase kinase binding [ISO]
- protein N-terminus binding [ISO]
- protein binding [IPI]
- protein kinase B binding [ISO]
- protein kinase binding [ISO]
- signal transducer activity [TAS]
- thioesterase binding [ISO]
- tumor necrosis factor receptor binding [ISO]
- ubiquitin conjugating enzyme binding [ISO]
- ubiquitin protein ligase activity [IDA, IMP]
- ubiquitin protein ligase binding [ISO]
- ubiquitin-protein transferase activity [IDA, ISO]
Gene Ontology Cellular Component
Mus musculus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
The E3 ligase Itch negatively regulates inflammatory signaling pathways by controlling the function of the ubiquitin-editing enzyme A20.
The ubiquitin-editing enzyme A20 is a critical negative regulator of inflammation and cytokine-mediated activation of the transcription factor NF-kappaB; however, little is known about the mechanisms of A20-mediated inactivation of signaling intermediates such as RIP1. Here we demonstrate that the regulatory molecule TAX1BP1 recruited the E3 ligase Itch to A20 via two 'PPXY' motifs. Itch was essential for the termination ... [more]
Nat. Immunol. Mar. 01, 2008; 9(3);254-62 [Pubmed: 18246070]
Throughput
- Low Throughput
Curated By
- BioGRID