An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.

Publication

Nuclear receptors TR2 and TR4 recruit multiple epigenetic transcriptional corepressors that associate specifically with the embryonic Î²-type globin promoters in differentiated adult erythroid cells.

Cui S, Kolodziej KE, Obara N, Amaral-Psarris A, Demmers J, Shi L, Engel JD, Grosveld F, Strouboulis J, Tanabe O

Nuclear receptors TR2 and TR4 (TR2/TR4) were previously shown to bind in vitro to direct repeat elements in the mouse and human embryonic and fetal Î²-type globin gene promoters and to play critical roles in the silencing of these genes. By chromatin immunoprecipitation (ChIP) we show that, in adult erythroid cells, TR2/TR4 bind to the embryonic Î²-type globin promoters but ... [more]

Mol. Cell. Biol. Aug. 01, 2011; 31(16);3298-311 [Pubmed: 21670149]

Throughput

Low Throughput

Additional Notes

figure 3.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
NR2C1 NR2C2	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Cui S (2011)	High	-	BioGRID	676446
NR2C2 NR2C1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Yu L (2018)	High	-	BioGRID	2607746
NR2C1 NR2C2	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Cui S (2011)	Low	-	BioGRID	676465

Curated By

BioGRID

Interaction Summary

NR2C1

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [TAS]protein binding [IPI]receptor activity [TAS]

Gene Ontology Cellular Component

NR2C2

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]protein heterodimerization activity [IDA]receptor activity [TAS]sequence-specific DNA binding [IDA]sequence-specific DNA binding transcription factor activity [IDA]transcription coactivator activity [TAS]