Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

SHARPIN negatively associates with TRAF2-mediated NFκB activation.

Liang Y

NFκB is an inducible transcriptional factor controlled by two principal signaling cascades and plays pivotal roles in diverse physiological processes including inflammation, apoptosis, oncogenesis, immunity, and development. Activation of NFκB signaling was detected in skin of SHAPRIN-deficient mice and can be diminished by an NFκB inhibitor. However, in vitro studies demonstrated that SHARPIN activates NFκB signaling by forming a linear ... [more]

PLoS ONE Aug. 11, 2011; 6(7);e21696 [Pubmed: 21829440]

Throughput

  • Low Throughput

Curated By

  • BioGRID