BAIT

APC

BTPS2, DP2, DP2.5, DP3, GS, PPP1R46
adenomatous polyposis coli
GO Process (21)
GO Function (8)
GO Component (12)
Homo sapiens
PREY

RPA2

REPA2, RP-A p32, RP-A p34, RPA32, RP4-547C9.3
replication protein A2, 32kDa
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Adenomatous polyposis coli protein regulates the cellular response to DNA replication stress.

Brocardo MG, Borowiec JA, Henderson BR

The adenomatous polyposis coli (APC) tumor suppressor traffics between nucleus and cytoplasm to perform distinct functions. Here we identify a specific role for APC in the DNA replication stress response. The silencing of APC caused an accumulation of asynchronous cells in early S phase and delayed S phase progression in cells released from hydroxyurea-mediated replication arrest. Immunoprecipitation assays revealed a ... [more]

Int. J. Biochem. Cell Biol. Sep. 01, 2011; 43(9);1354-64 [Pubmed: 21664290]

Throughput

  • Low Throughput

Additional Notes

  • figure 3A.
  • figure 6B.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RPA2 APC
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High-BioGRID
3633309

Curated By

  • BioGRID