INPPL1
Gene Ontology Biological Process
- actin filament organization [ISO]
- cellular lipid metabolic process [IMP]
- dephosphorylation [ISO]
- endochondral ossification [ISO]
- endocytosis [ISO]
- glucose metabolic process [IMP]
- inositol trisphosphate metabolic process [ISO]
- negative regulation of DNA replication [ISO]
- negative regulation of MAP kinase activity [ISO]
- negative regulation of cell proliferation [IDA]
- negative regulation of gene expression [IMP]
- negative regulation of insulin receptor signaling pathway [ISO]
- negative regulation of insulin-like growth factor receptor signaling pathway [ISO]
- negative regulation of neuron projection development [ISO]
- negative regulation of platelet-derived growth factor receptor signaling pathway [ISO]
- phosphatidylinositol biosynthetic process [IMP]
- post-embryonic development [IMP]
- response to insulin [IMP]
- ruffle assembly [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PPP2R1A
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Protein phosphatase 2A PR130/B''alpha1 subunit binds to the SH2 domain-containing inositol polyphosphate 5-phosphatase 2 and prevents epidermal growth factor (EGF)-induced EGF receptor degradation sustaining EGF-mediated signaling.
To elucidate novel cell biological functions of specific protein phosphatase 2A (PP2A) holoenzymes, we identified and biochemically characterized a complex between the Src homology 2 (SH2) domain-containing inositol polyphosphate 5-phosphatase 2 (SHIP2) and a PP2A holoenzyme comprising PR130/B''alpha1 as a regulatory subunit (PP2A(T130)) in several mammalian cell lines. PR130/B''alpha1 and SHIP2 partially colocalize in untreated HeLa cells, and both translocate ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID