An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Comparative analysis of cobalamin binding kinetics and ligand protection for intrinsic factor, transcobalamin, and haptocorrin.

Fedosov SN, Berglund L, Fedosova NU, Nexo E, Petersen TE

Changes in the absorbance spectrum of aquo-cobalamin (Cbl x OH(2)) revealed that its binding to transcobalamin (TC) is followed by slow conformational reorganization of the protein-ligand complex (Fedosov, S. N., Fedosova, N. U., Nexo, E., and Petersen, T. E. (2000) J. Biol. Chem. 275, 11791-11798). Two phases were also observed for TC when interacting with a Cbl-analogue cobinamide (Cbi), but ... [more]

J. Biol. Chem. Mar. 22, 2002; 277(12);9989-96 [Pubmed: 11788601]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

CBL

Gene Ontology Biological Process

Gene Ontology Molecular Function

SH3 domain binding [IPI]ephrin receptor binding [IPI]protein binding [IPI]sequence-specific DNA binding transcription factor activity [TAS]ubiquitin-protein transferase activity [TAS]

Gene Ontology Cellular Component

TCN1