An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Mechanistic rationale for inhibition of poly(ADP-ribose) polymerase in ETS gene fusion-positive prostate cancer.

Brenner JC, Ateeq B, Li Y, Yocum AK, Cao Q, Asangani IA, Patel S, Wang X, Liang H, Yu J, Palanisamy N, Siddiqui J, Yan W, Cao X, Mehra R, Sabolch A, Basrur V, Lonigro RJ, Yang J, Tomlins SA, Maher CA, Elenitoba-Johnson KS, Hussain M, Navone NM, Pienta KJ, Varambally S, Feng FY, Chinnaiyan AM

Recurrent fusions of ETS genes are considered driving mutations in a diverse array of cancers, including Ewing's sarcoma, acute myeloid leukemia, and prostate cancer. We investigate the mechanisms by which ETS fusions mediate their effects, and find that the product of the predominant ETS gene fusion, TMPRSS2:ERG, interacts in a DNA-independent manner with the enzyme poly (ADP-ribose) polymerase 1 (PARP1) ... [more]

Cancer Cell May. 17, 2011; 19(5);664-78 [Pubmed: 21575865]

Throughput

High Throughput

Additional Notes

figure 1, table S1.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
ERG PARP1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Wang S (2014)	High	-	BioGRID	935589
ERG PARP1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Brenner JC (2011)	Low	-	BioGRID	677539
ERG PARP1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Wang S (2014)	High	-	BioGRID	935639

Curated By

BioGRID

Interaction Summary

ERG

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [TAS]protein binding [IPI]sequence-specific DNA binding RNA polymerase II transcription factor activity [IBA]signal transducer activity [TAS]

Gene Ontology Cellular Component

PARP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

NAD+ ADP-ribosyltransferase activity [IDA, TAS]enzyme binding [IPI]identical protein binding [IPI]poly(A) RNA binding [IDA]protein N-terminus binding [IPI]protein binding [IPI]transcription factor binding [IPI]