BAIT

RHBDL3

RHBDL4, VRHO

rhomboid, veinlet-like 3 (Drosophila)

GO Process (0)

GO Function (0)

GO Component (0)

CRISPR Database VEGA HGNC Alliance of Genome Resources Entrez Gene RefSeq UniprotKB Ensembl HPRD

Homo sapiens

PREY

PTCRA

PT-ALPHA, PTA, RP3-475N16.3

pre T-cell antigen receptor alpha

GO Process (0)

GO Function (1)

GO Component (0)

Gene Ontology Molecular Function

protein binding [IPI]

CRISPR Database HGNC Alliance of Genome Resources VEGA OMIM Entrez Gene RefSeq UniprotKB Ensembl HPRD

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Ubiquitin-Dependent Intramembrane Rhomboid Protease Promotes ERAD of Membrane Proteins.

Fleig L, Bergbold N, Sahasrabudhe P, Geiger B, Kaltak L, Lemberg MK

The ER-associated degradation (ERAD) pathway serves as an important cellular safeguard by directing incorrectly folded and unassembled proteins from the ER to the proteasome. Still, however, little is known about the components mediating ERAD ofÂ membrane proteins. Here we show that the evolutionary conserved rhomboid family protein RHBDL4 is a ubiquitin-dependent ER-resident intramembrane protease that is upregulated upon ER stress. RHBDL4 ... [more]

Unknown Jul. 10, 2012; 0(0); [Pubmed: 22795130]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary