An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Human ISG15 conjugation targets both IFN-induced and constitutively expressed proteins functioning in diverse cellular pathways.

Zhao C, Denison C, Huibregtse JM, Gygi S, Krug RM

IFN-alpha/beta plays an essential role in innate immunity against viral and bacterial infection. Among the proteins induced by IFN-alpha/beta are the ubiquitin-like ISG15 protein and its E1- (Ube1L) and E2- (UbcH8) conjugating enzymes, leading to the conjugation of ISG15 to cellular proteins. It is likely that ISG15 conjugation plays an important role in antiviral response because a human virus, influenza ... [more]

Proc. Natl. Acad. Sci. U.S.A. Jul. 19, 2005; 102(29);10200-5 [Pubmed: 16009940]

Throughput

High Throughput

Ontology Terms

cell line: hela cell (BTO:0000567) [cervical adenocarcinoma (DOID:3702)]

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
ISG15 CCT3	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Bhushan J (2020)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

ISG15

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

CCT3