ISG15
Gene Ontology Biological Process
- ISG15-protein conjugation [IDA, IMP]
- cytokine-mediated signaling pathway [TAS]
- defense response to bacterium [IMP]
- defense response to virus [IMP]
- innate immune response [TAS]
- negative regulation of protein ubiquitination [IDA]
- negative regulation of type I interferon production [TAS]
- negative regulation of viral genome replication [IMP]
- regulation of interferon-gamma production [IMP]
- response to type I interferon [IDA]
- type I interferon signaling pathway [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
MSN
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Human ISG15 conjugation targets both IFN-induced and constitutively expressed proteins functioning in diverse cellular pathways.
IFN-alpha/beta plays an essential role in innate immunity against viral and bacterial infection. Among the proteins induced by IFN-alpha/beta are the ubiquitin-like ISG15 protein and its E1- (Ube1L) and E2- (UbcH8) conjugating enzymes, leading to the conjugation of ISG15 to cellular proteins. It is likely that ISG15 conjugation plays an important role in antiviral response because a human virus, influenza ... [more]
Throughput
- High Throughput
Ontology Terms
- cell line: hela cell (BTO:0000567) [cervical adenocarcinoma (DOID:3702)]
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| MSN ISG15 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 678267 |
Curated By
- BioGRID