An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

In vivo RNAi screen reveals neddylation genes as novel regulators of Hedgehog signaling.

Du J, Zhang J, Su Y, Liu M, Ospina JK, Yang S, Zhu AJ

Hedgehog (Hh) signaling is highly conserved in all metazoan animals and plays critical roles in many developmental processes. Dysregulation of the Hh signaling cascade has been implicated in many diseases, including cancer. Although key components of the Hh pathway have been identified, significant gaps remain in our understanding of the regulation of individual Hh signaling molecules. Here, we report the ... [more]

PLoS ONE Sep. 21, 2011; 6(9);e24168 [Pubmed: 21931660]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
UBA3 APP-BP1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Giot L (2003)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

APP-BP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

NEDD8 activating enzyme activity [IDA, ISS]protein binding [IPI]protein heterodimerization activity [ISS]ubiquitin activating enzyme activity [ISS]

Gene Ontology Cellular Component

UBA3