BAIT

UBQLN2

ALS15, CHAP1, DSK2, N4BP4, PLIC2, HRIHFB2157
ubiquilin 2
Autophagy Project
UPS Project
GO Process (0)
GO Function (1)
GO Component (2)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

EPN2

EHB21
epsin 2
UPS Project
GO Process (0)
GO Function (0)
GO Component (3)

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The ubiquitin-like protein PLIC-2 is a negative regulator of G protein-coupled receptor endocytosis.

N'Diaye EN, Hanyaloglu AC, Kajihara KK, Puthenveedu MA, Wu P, von Zastrow M, Brown EJ

The activity of many signaling receptors is regulated by their endocytosis via clathrin-coated pits (CCPs). For G protein-coupled receptors (GPCRs), recruitment of the adaptor protein arrestin to activated receptors is thought to be sufficient to drive GPCR clustering in CCPs and subsequent endocytosis. We have identified an unprecedented role for the ubiquitin-like protein PLIC-2 as a negative regulator of GPCR ... [more]

Mol. Biol. Cell Mar. 01, 2008; 19(3);1252-60 [Pubmed: 18199683]

Throughput

  • Low Throughput

Curated By

  • BioGRID