Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Dynamin interacts with members of the sumoylation machinery.

Mishra RK, Jatiani SS, Kumar A, Simhadri VR, Hosur RV, Mittal R

Dynamin is a GTP-binding protein whose oligomerization-dependent assembly around the necks of lipid vesicles mediates their scission from parent membranes. Dynamin is thus directly involved in the regulation of endocytosis. Sumoylation is a post-translational protein modification whereby the ubiquitin-like modifier Sumo is covalently attached to lysine residues on target proteins by a process requiring the concerted action of an activating ... [more]

J. Biol. Chem. Jul. 23, 2004; 279(30);31445-54 [Pubmed: 15123615]

Throughput

  • High Throughput|Low Throughput

Curated By

  • BioGRID