IRF1
Gene Ontology Biological Process
- CD8-positive, alpha-beta T cell differentiation [IMP]
- I-kappaB kinase/NF-kappaB signaling [ISO]
- apoptotic process [ISO]
- cell cycle arrest [ISO]
- cellular response to interferon-beta [ISO]
- cellular response to interleukin-1 [ISO]
- cellular response to peptide hormone stimulus [ISO]
- cellular response to tumor necrosis factor [ISO]
- defense response to virus [IMP, ISO]
- interferon-gamma-mediated signaling pathway [IMP]
- negative regulation of T-helper 2 cell differentiation [TAS]
- negative regulation of regulatory T cell differentiation [IMP]
- negative regulation of transcription, DNA-templated [IDA, ISO]
- negative regulation of tyrosine phosphorylation of Stat1 protein [ISO]
- positive regulation of T-helper 1 cell differentiation [TAS]
- positive regulation of cell death [ISO]
- positive regulation of interferon-beta production [ISO]
- positive regulation of interleukin-12 biosynthetic process [IDA, IMP]
- positive regulation of natural killer cell differentiation [TAS]
- positive regulation of transcription from RNA polymerase II promoter [IDA, ISO]
- positive regulation of transcription, DNA-templated [IDA, IMP, ISO]
- positive regulation of type I interferon production [IMP]
- regulation of CD8-positive, alpha-beta T cell proliferation [IMP]
- regulation of MyD88-dependent toll-like receptor signaling pathway [IMP]
- regulation of adaptive immune response [TAS]
- regulation of gene expression [IMP]
- regulation of innate immune response [TAS]
- response to growth hormone [ISO]
- transcription from RNA polymerase II promoter [ISO]
Gene Ontology Molecular Function- DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- protein binding [IPI]
- sequence-specific DNA binding [IDA, ISO]
- sequence-specific DNA binding transcription factor activity [IDA, ISO]
- DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- protein binding [IPI]
- sequence-specific DNA binding [IDA, ISO]
- sequence-specific DNA binding transcription factor activity [IDA, ISO]
PIAS3
Gene Ontology Biological Process
Gene Ontology Molecular Function
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
PIAS3 induces SUMO-1 modification and transcriptional repression of IRF-1.
Interferon regulatory factor-1 (IRF-1) is a transcriptional activator that is involved in interferon response, regulation of cell growth and oncogenesis. To try to identify the molecules that regulate the function of IRF-1, we performed yeast two-hybrid screening and isolated protein inhibitor of activated STAT3 (PIAS3) as an IRF-1-binding protein. This protein was also found to bind with small ubiquitin-related modifier-1 ... [more]
Throughput
- High Throughput|Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| IRF1 PIAS3 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID