Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Importin-11, a nuclear import receptor for the ubiquitin-conjugating enzyme, UbcM2.

Plafker SM, Macara IG

Importins are members of a family of transport receptors (karyopherins) that mediate the nucleocytoplasmic transport of protein and RNA cargoes. We identified importin-11 as a potential new human member of this family, on the basis of limited similarity to the Saccharomyces cerevisiae protein, Lph2p, and cloned the complete open reading frame. Importin-11 interacts with the Ran GTPase, and constitutively shuttles ... [more]

EMBO J. Oct. 16, 2000; 19(20);5502-13 [Pubmed: 11032817]

Throughput

  • Low Throughput

Curated By

  • BioGRID