RGS14
Gene Ontology Biological Process
- cell division [IMP]
- chromosome segregation [ISS]
- intracellular signal transduction [ISS]
- learning [ISS]
- long-term memory [ISS]
- long-term synaptic potentiation [ISS]
- negative regulation of ERK1 and ERK2 cascade [ISS]
- negative regulation of MAP kinase activity [ISS]
- negative regulation of synaptic plasticity [ISS]
- nucleocytoplasmic transport [ISS]
- platelet-derived growth factor receptor signaling pathway [ISS]
- positive regulation of GTPase activity [IBA, IDA, TAS]
- positive regulation of neurogenesis [ISS]
- regulation of DNA-templated transcription in response to stress [ISS]
- regulation of G-protein coupled receptor protein signaling pathway [TAS]
- response to oxidative stress [ISS]
- spindle organization [IMP]
- visual learning [ISS]
- zygote asymmetric cell division [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
GNA11
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Structure-based protocol for identifying mutations that enhance protein-protein binding affinities.
The ability to manipulate protein binding affinities is important for the development of proteins as biosensors, industrial reagents, and therapeutics. We have developed a structure-based method to rationally predict single mutations at protein-protein interfaces that enhance binding affinities. The protocol is based on the premise that increasing buried hydrophobic surface area and/or reducing buried hydrophilic surface area will generally lead ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID