BAIT

SKP2

FBL1, FBXL1, FLB1, p45
S-phase kinase-associated protein 2, E3 ubiquitin protein ligase
UPS Project
Homo sapiens
PREY

KPNA6

IPOA7, KPNA7, RP4-622L5.1
karyopherin alpha 6 (importin alpha 7)
GO Process (1)
GO Function (0)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Phosphorylation by Akt1 promotes cytoplasmic localization of Skp2 and impairs APCCdh1-mediated Skp2 destruction.

Gao D, Inuzuka H, Tseng A, Chin RY, Toker A, Wei W

Deregulated Skp2 function promotes cell transformation, and this is consistent with observations of Skp2 overexpression in many human cancers. However, the mechanisms underlying elevated Skp2 expression are still unknown. Here we show that the serine/threonine protein kinase Akt1, but not Akt2, directly controls Skp2 stability by a mechanism that involves degradation by the APC-Cdh1 ubiquitin ligase complex. We show further ... [more]

Nat. Cell Biol. Apr. 01, 2009; 11(4);397-408 [Pubmed: 19270695]

Throughput

  • Low Throughput

Additional Notes

  • figure 7f.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SKP2 KPNA6
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
829894
KPNA6 SKP2
Protein-peptide
Protein-peptide

An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments.

Low-BioGRID
829898

Curated By

  • BioGRID