BAIT

CPEB1

CPE-BP1, CPEB, CPEB-1, hCPEB-1
cytoplasmic polyadenylation element binding protein 1
Homo sapiens
PREY

CPEB1

CPE-BP1, CPEB, CPEB-1, hCPEB-1
cytoplasmic polyadenylation element binding protein 1
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Transient CPEB dimerization and translational control.

Lin CL, Huang YT, Richter JD

During oocyte development, the cytoplasmic polyadenylation element-binding protein (CPEB) nucleates a set of factors on mRNA that controls cytoplasmic polyadenylation and translation. The regulation of polyadenylation is mediated in part through serial phosphorylations of CPEB, which control both the dynamic integrity of the cytoplasmic polyadenylation apparatus and CPEB stability, events necessary for meiotic progression. Because the precise stoichiometry between CPEB ... [more]

RNA May. 01, 2012; 18(5);1050-61 [Pubmed: 22456264]

Throughput

  • Low Throughput

Curated By

  • BioGRID