BAIT
NBR1
mKIAA0049, RP23-382C19.1
neighbor of Brca1 gene 1
GO Process (5)
GO Function (3)
GO Component (6)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
UBB
AL033289, Rps27a, Uba52, Ubb2, Ubc, RP23-234K24.1
ubiquitin B
GO Process (16)
GO Function (0)
GO Component (4)
Gene Ontology Biological Process
- adipose tissue development [IMP]
- energy homeostasis [IMP]
- fat pad development [IMP]
- female gonad development [IMP]
- female meiosis I [IMP]
- hypothalamus gonadotrophin-releasing hormone neuron development [IMP]
- male gonad development [IMP]
- male meiosis I [IMP]
- mitochondrion transport along microtubule [ISO]
- neuron projection morphogenesis [ISO]
- positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator [ISO]
- regulation of mitochondrial membrane potential [ISO]
- regulation of neuron death [ISO]
- regulation of proteasomal protein catabolic process [ISO]
- seminiferous tubule development [IMP]
- ubiquitin homeostasis [IGI, IMP]
Gene Ontology Cellular Component
Mus musculus
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Interactions with LC3 and polyubiquitin chains link nbr1 to autophagic protein turnover.
Nbr1, a ubiquitous kinase scaffold protein, contains a PB1, and a ubiquitin-associated (UBA) domain. We show here that the nbr1 UBA domain binds to lysine-48 and -63 linked polyubiquitin-B chains. Nbr1 also binds to the autophagic effector protein LC3-A via a novel binding site. Ubiquitin-binding, but not PB1-mediated p62/SQSTM1 interaction, is required to target nbr1 to LC3 and polyubiquitin-positive bodies. ... [more]
FEBS Lett. Jun. 18, 2009; 583(12);1846-52 [Pubmed: 19427866]
Throughput
- Low Throughput
Additional Notes
- #LPPI
- Likely protein-protein interaction
Curated By
- BioGRID