BAIT

ZP3

ZP3A, ZP3B, ZPC, Zp-3
zona pellucida glycoprotein 3 (sperm receptor)
GO Process (32)
GO Function (6)
GO Component (12)

Gene Ontology Biological Process

Homo sapiens
PREY

UBAP2L

NICE-4, RP11-205M9.4
ubiquitin associated protein 2-like
GO Process (1)
GO Function (2)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Identification of human sperm proteins that interact with human zona pellucida3 (ZP3) using yeast two-hybrid system.

Naz RK, Dhandapani L

Sperm proteins that interact with zona pellucida 3 (ZP3) have not been clearly identified in humans. In the present study, the yeast two-hybrid (Y2H) system was used to identify human sperm proteins that interact with human ZP3. Human ZP3 cDNA was cloned into pAS2-1 yeast vector and used as bait to find reactive proteins in the human testis cDNA library. ... [more]

J. Reprod. Immunol. Jan. 01, 2010; 84(1);24-31 [Pubmed: 19945174]

Throughput

  • High Throughput|Low Throughput

Additional Notes

  • mammalian two-hybrid system.
  • table 2.

Curated By

  • BioGRID