BAIT
ZP3
ZP3A, ZP3B, ZPC, Zp-3
zona pellucida glycoprotein 3 (sperm receptor)
GO Process (32)
GO Function (6)
GO Component (12)
Gene Ontology Biological Process
- binding of sperm to zona pellucida [IDA, IGI, IMP, TAS]
- blastocyst formation [ISS]
- calcium ion transmembrane transport [IDA]
- egg coat formation [ISS]
- humoral immune response mediated by circulating immunoglobulin [ISS]
- intracellular protein transport [ISS]
- intracellular signal transduction [IMP]
- manganese ion transmembrane transport [ISS]
- manganese ion transport [ISS]
- multicellular organism reproduction [TAS]
- negative regulation of binding of sperm to zona pellucida [IDA]
- negative regulation of transcription, DNA-templated [ISS]
- oocyte development [ISS]
- phosphatidylinositol-mediated signaling [ISS]
- positive regulation of T cell proliferation [ISS]
- positive regulation of acrosomal vesicle exocytosis [IDA, IMP]
- positive regulation of acrosome reaction [IDA]
- positive regulation of antral ovarian follicle growth [ISS]
- positive regulation of calcium ion import [IDA]
- positive regulation of humoral immune response [IDA]
- positive regulation of inflammatory response [ISS]
- positive regulation of interferon-gamma production [ISS]
- positive regulation of interleukin-4 production [ISS]
- positive regulation of leukocyte migration [ISS]
- positive regulation of ovarian follicle development [ISS]
- positive regulation of phosphatidylinositol biosynthetic process [ISS]
- positive regulation of protein kinase B signaling [ISS]
- positive regulation of protein kinase activity [IDA]
- positive regulation of transcription, DNA-templated [ISS]
- positive regulation of type IV hypersensitivity [ISS]
- protein kinase C signaling [IMP]
- single fertilization [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
PCDH17
PCDH68, PCH68
protocadherin 17
GO Process (0)
GO Function (1)
GO Component (0)
Gene Ontology Molecular Function
Homo sapiens
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Identification of human sperm proteins that interact with human zona pellucida3 (ZP3) using yeast two-hybrid system.
Sperm proteins that interact with zona pellucida 3 (ZP3) have not been clearly identified in humans. In the present study, the yeast two-hybrid (Y2H) system was used to identify human sperm proteins that interact with human ZP3. Human ZP3 cDNA was cloned into pAS2-1 yeast vector and used as bait to find reactive proteins in the human testis cDNA library. ... [more]
J. Reprod. Immunol. Jan. 01, 2010; 84(1);24-31 [Pubmed: 19945174]
Throughput
- High Throughput|Low Throughput
Additional Notes
- mammalian two-hybrid system.
- table 2.
Curated By
- BioGRID