An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Ubiquitin protein ligase Nedd4 binds to connexin43 by a phosphorylation-modulated process.

Leykauf K, Salek M, Bomke J, Frech M, Lehmann WD, Duerst M, Alonso A

Connexin43 is degraded by the proteasomal as well as the lysosomal pathway with ubiquitin playing a role in both degradation pathways. So far, no ubiquitin protein ligase has been identified for any of the connexins. By using pull-down assays, here we show binding of a ubiquitin protein ligase, Nedd4, to the C-terminus of connexin43. This observation was confirmed in vivo ... [more]

J. Cell. Sci. Sep. 01, 2006; 119(0);3634-42 [Pubmed: 16931598]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
GJA1 NEDD4	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Liao CK (2013)	Low	-	BioGRID	-
GJA1 NEDD4	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Martins-Marques T (2015)	Low	-	BioGRID	1509655
GJA1 NEDD4	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Mollerup S (2011)	Low	-	BioGRID	728706
NEDD4 GJA1	Co-crystal Structure Co-crystal Structure Interaction directly demonstrated at the atomic level by X-ray crystallography. Also used for NMR or Electron Microscopy (EM) structures. If there is no obvious bait-hit directionality to the interaction involving 3 or more proteins, then the co-crystallized proteins should be listed as a complex.	Spagnol G (2016)	Low	-	BioGRID	1516476
GJA1 NEDD4	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Leykauf K (2006)	Low	-	BioGRID	-
NEDD4 GJA1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Spagnol G (2016)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

GJA1

Gene Ontology Biological Process

Gene Ontology Molecular Function

PDZ domain binding [IPI]SH3 domain binding [IPI]beta-tubulin binding [ISO]connexin binding [IPI]gap junction channel activity [IDA, ISO]protein binding [IPI]protein domain specific binding [IDA]receptor binding [ISO]scaffold protein binding [ISO]signal transducer activity [ISO]

Gene Ontology Cellular Component

NEDD4