An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Mediation of Af4 protein function in the cerebellum by Siah proteins.

Oliver PL, Bitoun E, Clark J, Jones EL, Davies KE

We have established that the gene AF4, which had long been recognized as disrupted in childhood leukemia, also plays a role in the CNS. Af4 is mutated in the robotic mouse that is characterized by ataxia and Purkinje cell loss. To determine the molecular basis of this mutation, we carried out a yeast two-hybrid screen and show that Af4 binds ... [more]

Proc. Natl. Acad. Sci. U.S.A. Oct. 12, 2004; 101(41);14901-6 [Pubmed: 15459319]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SIAH1A AFF2	Protein-peptide Protein-peptide An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments.	Oliver PL (2004)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

SIAH1A

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein C-terminus binding [ISO]protein binding [IPI]protein homodimerization activity [IPI]ubiquitin protein ligase activity [ISO, TAS]ubiquitin-protein transferase activity [IDA, ISO]zinc ion binding [IDA, ISO]

Gene Ontology Cellular Component

AFF2