An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

p619, a giant protein related to the chromosome condensation regulator RCC1, stimulates guanine nucleotide exchange on ARF1 and Rab proteins.

Rosa JL, Casaroli-Marano RP, Buckler AJ, Vilaro S, Barbacid M

We report the identification of a novel human gene, designated p619, that encodes a polypeptide of 4861 amino acid residues, one of the largest human proteins known to date. The p619 protein contains two regions of seven internal repeats highly related to the cell cycle regulator RCC1, a guanine nucleotide exchange factor for the small GTP binding protein, Ran. In ... [more]

EMBO J. Aug. 15, 1996; 15(16);4262-73 [Pubmed: 8861955]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

HERC1

Gene Ontology Biological Process

Gene Ontology Molecular Function

ARF guanyl-nucleotide exchange factor activity [TAS]

Gene Ontology Cellular Component

ARF1