TBK1
Gene Ontology Biological Process
- activation of innate immune response [IMP]
- defense response to Gram-positive bacterium [IMP]
- dendritic cell proliferation [IGI]
- negative regulation of gene expression [IMP]
- positive regulation of interferon-alpha production [ISO]
- positive regulation of interferon-beta biosynthetic process [IMP]
- positive regulation of interferon-beta production [ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
Gene Ontology Molecular Function
IRF3
Gene Ontology Biological Process
- cellular response to lipopolysaccharide [IDA]
- defense response to virus [IMP]
- lipopolysaccharide-mediated signaling pathway [IDA]
- negative regulation of defense response to virus by host [IDA]
- negative regulation of interferon-beta biosynthetic process [IDA]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [ISO]
- positive regulation of cytokine secretion [ISO]
- positive regulation of interferon-alpha production [IMP]
- positive regulation of interferon-beta production [IMP, TAS]
- positive regulation of type I interferon-mediated signaling pathway [IMP]
- programmed necrotic cell death [IMP]
- response to bacterium [IDA]
- response to exogenous dsRNA [IDA]
- response to lipopolysaccharide [IDA]
- type I interferon biosynthetic process [IGI]
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Polyubiquitin binding to optineurin is required for optimal activation of TANK-binding kinase 1 and production of interferon β.
TANK-binding kinase (TBK1) is essential for transcription of the interferon (IFN) β gene in response to lipopolysaccharide (LPS) and double-stranded RNA, but the molecular mechanisms that underlie the activation of TBK1 are incompletely understood. Previously, we identified the NF-κB essential modulator (NEMO)-related polyubiquitin-binding protein, optineurin (OPTN), as a novel binding partner of TBK1. To determine whether the ubiquitin-binding function of ... [more]
Throughput
- Low Throughput
Additional Notes
- figure 4A.
- figure 6A.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| TBK1 IRF3 | Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation. | Low | - | BioGRID | 680451 |
Curated By
- BioGRID