An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Rab GTPase-activating proteins in autophagy: regulation of endocytic and autophagy pathways by direct binding to human ATG8 modifiers.

Popovic D, Akutsu M, Novak I, Harper JW, Behrends C, Dikic I

Autophagy is an evolutionarily conserved degradation pathway characterized by dynamic rearrangement of membranes that sequester cytoplasm, protein aggregates, organelles, and pathogens for delivery to the vacuole and lysosome, respectively. The ability of autophagosomal membranes to act selectively toward specific cargo is dependent on the small ubiquitin-like modifier ATG8/LC3 and the LC3-interacting region (LIR) present in autophagy receptors. Here, we describe ... [more]

Mol. Cell. Biol. May. 01, 2012; 32(9);1733-44 [Pubmed: 22354992]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
MAP1LC3A TBC1D16	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Popovic D (2012)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

MAP1LC3A

Gene Ontology Biological Process

Gene Ontology Molecular Function

GABA receptor binding [IBA]microtubule binding [IBA]phosphatidylethanolamine binding [IDA]phospholipid binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

TBC1D16