BAIT
ACD
PIP1, PTOP, TINT1, TPP1
adrenocortical dysplasia homolog (mouse)
GO Process (9)
GO Function (2)
GO Component (5)
Gene Ontology Biological Process
- intracellular protein transport [IMP]
- negative regulation of telomere maintenance via telomerase [IGI, IMP]
- positive regulation of single-stranded telomeric DNA binding [IDA]
- positive regulation of telomerase activity [IDA]
- protection from non-homologous end joining at telomere [ISS]
- protein localization to chromosome, telomeric region [IMP]
- telomere assembly [IMP]
- telomere capping [IGI, NAS]
- telomere maintenance [IDA, TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
GAPDH
G3PD, GAPD, HEL-S-162eP, CDABP0047
glyceraldehyde-3-phosphate dehydrogenase
GO Process (11)
GO Function (5)
GO Component (13)
Gene Ontology Biological Process
- carbohydrate metabolic process [TAS]
- cellular response to interferon-gamma [IDA]
- gluconeogenesis [TAS]
- glucose metabolic process [TAS]
- glycolytic process [TAS]
- microtubule cytoskeleton organization [ISS]
- negative regulation of translation [IDA, IMP]
- neuron apoptotic process [ISS]
- peptidyl-cysteine S-trans-nitrosylation [ISS]
- protein stabilization [ISS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- GAIT complex [IDA]
- cytoplasm [IDA, ISS]
- cytosol [IDA, ISS, TAS]
- extracellular vesicular exosome [IDA]
- intracellular membrane-bounded organelle [IDA]
- lipid particle [IDA]
- membrane [IDA]
- microtubule cytoskeleton [ISS]
- nuclear membrane [IDA]
- nucleus [IDA, ISS]
- plasma membrane [IDA]
- ribonucleoprotein complex [IDA]
- vesicle [IDA]
Homo sapiens
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Genome-wide YFP fluorescence complementation screen identifies new regulators for telomere signaling in human cells.
Detection of low-affinity or transient interactions can be a bottleneck in our understanding of signaling networks. To address this problem, we developed an arrayed screening strategy based on protein complementation to systematically investigate protein-protein interactions in live human cells, and performed a large-scale screen for regulators of telomeres. Maintenance of vertebrate telomeres requires the concerted action of members of the ... [more]
Mol. Cell Proteomics Feb. 01, 2011; 10(2);M110.001628 [Pubmed: 21044950]
Throughput
- High Throughput
Additional Notes
- BifC
Curated By
- BioGRID