PREY

DCX

DBCN, DC, LISX, SCLH, XLIS, RP5-914P14.1
doublecortin
GO Process (4)
GO Function (3)
GO Component (4)
Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Genome-wide YFP fluorescence complementation screen identifies new regulators for telomere signaling in human cells.

Lee OH, Kim H, He Q, Baek HJ, Yang D, Chen LY, Liang J, Chae HK, Safari A, Liu D, Songyang Z

Detection of low-affinity or transient interactions can be a bottleneck in our understanding of signaling networks. To address this problem, we developed an arrayed screening strategy based on protein complementation to systematically investigate protein-protein interactions in live human cells, and performed a large-scale screen for regulators of telomeres. Maintenance of vertebrate telomeres requires the concerted action of members of the ... [more]

Mol. Cell Proteomics Feb. 01, 2011; 10(2);M110.001628 [Pubmed: 21044950]

Throughput

  • High Throughput

Additional Notes

  • BifC

Curated By

  • BioGRID