BAIT

LAMP2

CD107b, LAMP-2, LAMPB, LGP110
lysosomal-associated membrane protein 2
Alzheimer's Disease Project
Autophagy Project
Homo sapiens
PREY

UCHL1

HEL-117, NDGOA, PARK5, PGP 9.5, PGP9.5, PGP95, Uch-L1
ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
UPS Project
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Aberrant interaction between Parkinson disease-associated mutant UCH-L1 and the lysosomal receptor for chaperone-mediated autophagy.

Kabuta T, Furuta A, Aoki S, Furuta K, Wada K

Parkinson disease (PD) is the most common neurodegenerative movement disorder. An increase in the amount of alpha-synuclein protein could constitute a cause of PD. Alpha-synuclein is degraded at least partly by chaperone-mediated autophagy (CMA). The I93M mutation in ubiquitin C-terminal hydrolase L1 (UCH-L1) is associated with familial PD. However, the relationship between alpha-synuclein and UCH-L1 in the pathogenesis of PD ... [more]

J. Biol. Chem. Aug. 29, 2008; 283(35);23731-8 [Pubmed: 18550537]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
UCHL1 LAMP2
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
-

Curated By

  • BioGRID