DPM3
Gene Ontology Biological Process
- C-terminal protein lipidation [TAS]
- GPI anchor biosynthetic process [IDA, TAS]
- carbohydrate metabolic process [NAS]
- cellular protein metabolic process [TAS]
- dolichol-linked oligosaccharide biosynthetic process [TAS]
- post-translational protein modification [TAS]
- protein C-linked glycosylation via 2'-alpha-mannosyl-L-tryptophan [TAS]
- protein N-linked glycosylation via asparagine [TAS]
- protein O-linked mannosylation [TAS]
- protein mannosylation [TAS]
- regulation of protein stability [IPI]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
DPM1
Gene Ontology Biological Process
- C-terminal protein lipidation [TAS]
- GPI anchor biosynthetic process [IDA]
- cellular protein metabolic process [TAS]
- dolichol metabolic process [IDA]
- dolichol-linked oligosaccharide biosynthetic process [TAS]
- post-translational protein modification [TAS]
- protein N-linked glycosylation via asparagine [TAS]
- protein O-linked mannosylation [IDA]
- protein mannosylation [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
DPM1, the catalytic subunit of dolichol-phosphate mannose synthase, is tethered to and stabilized on the endoplasmic reticulum membrane by DPM3.
Dolichol-phosphate mannose (DPM) synthase is required for synthesis of the glycosylphosphatidylinositol (GPI) anchor, N-glycan precursor, protein O-mannose, and C-mannose. We previously identified DPM3, the third component of this enzyme, which was co-purified with DPM1 and DPM2. Here, we have established mutant Chinese hamster ovary (CHO) 2.38 cells that were defective in DPM3. CHO2.38 cells were negative for GPI-anchored proteins, and ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| DPM1 DPM3 | Co-purification Co-purification An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps. | Low | - | BioGRID | - |
Curated By
- BioGRID