PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

RNF41 (Nrdp1) controls type 1 cytokine receptor degradation and ectodomain shedding.

Wauman J, De Ceuninck L, Vanderroost N, Lievens S, Tavernier J

Cytokines, such as interferons, erythropoietin, leptin and most interleukins, signal through type 1 cytokine receptors and activate the canonical JAK-STAT pathway. Aberrant cytokine signalling underlies numerous pathologies and adequate, temporary receptor activation is therefore under tight control. Negative-feedback mechanisms are very well studied, but cellular sensitivity also depends on the number of receptors exposed at the cell surface. This is ... [more]

J. Cell. Sci. Mar. 15, 2011; 124(0);921-32 [Pubmed: 21378310]

Throughput

  • Low Throughput

Additional Notes

  • MAPPIT

Curated By

  • BioGRID