USP2
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
CRY1
Gene Ontology Biological Process
- DNA damage induced protein phosphorylation [IDA]
- circadian regulation of gene expression [IGI, IMP]
- circadian rhythm [IDA, IMP]
- entrainment of circadian clock by photoperiod [IMP]
- gluconeogenesis [IMP]
- glucose homeostasis [IGI]
- lipid storage [IGI]
- negative regulation of G-protein coupled receptor protein signaling pathway [IMP]
- negative regulation of circadian rhythm [IDA, IMP]
- negative regulation of glucocorticoid receptor signaling pathway [IDA, IGI]
- negative regulation of glucocorticoid secretion [IGI]
- negative regulation of protein ubiquitination [IDA]
- negative regulation of transcription from RNA polymerase II promoter [IMP, ISO]
- negative regulation of transcription, DNA-templated [IDA, IGI, ISO]
- regulation of DNA damage checkpoint [IDA]
- regulation of circadian rhythm [IMP]
- response to glucagon [IMP]
- response to insulin [IGI]
Gene Ontology Molecular Function- core promoter binding [IDA]
- core promoter sequence-specific DNA binding [IDA]
- double-stranded DNA binding [IDA, ISO]
- histone deacetylase binding [IPI]
- kinase binding [IPI]
- nuclear hormone receptor binding [IPI, ISO]
- phosphatase binding [ISO]
- protein binding [IPI]
- protein kinase binding [IPI]
- transcription factor binding [IPI]
- transcription factor binding transcription factor activity [ISO]
- ubiquitin binding [IDA]
- core promoter binding [IDA]
- core promoter sequence-specific DNA binding [IDA]
- double-stranded DNA binding [IDA, ISO]
- histone deacetylase binding [IPI]
- kinase binding [IPI]
- nuclear hormone receptor binding [IPI, ISO]
- phosphatase binding [ISO]
- protein binding [IPI]
- protein kinase binding [IPI]
- transcription factor binding [IPI]
- transcription factor binding transcription factor activity [ISO]
- ubiquitin binding [IDA]
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
The de-ubiquitinylating enzyme, USP2, is associated with the circadian clockwork and regulates its sensitivity to light.
We have identified a novel component of the circadian clock that regulates its sensitivity to light at the evening light to dark transition. USP2 (Ubiquitin Specific Protease 2), which de-ubiquitinylates and stabilizes target proteins, is rhythmically expressed in multiple tissues including the SCN. We have developed a knockout model of USP2 and found that exposure to low irradiance light at ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CRY1 USP2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| USP2 CRY1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| CRY1 USP2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID